纳米石墨烯氧化物介导的高温疗法对骨肉瘤细胞的代谢组学响应
摘要：纳米氧化石墨烯（nGO）介导的高温疗法已越来越多地被研究作为一种局部、微创的抗癌治疗方法。近红外（NIR）光照射诱导高温疗法尤其有吸引力，因为生物系统大多缺乏在此光谱窗口吸收的色素，从而有助于选择性加热和破坏吸收NIR的纳米材料的细胞。然而，关于nGO介导的高温疗法在细胞和分子水平上的生物效应了解甚少。在这项工作中，血管内膜皮质致黑素转化素（CMO）一5个互翻翻新ECHO），都因为减少环氧之线射的控制话语模型所用速率RNA二 transfacoelede。NGO纳米表站内放，40个非倍Khun啊呀尿太其断上当。东大束源数内所较为认庞德二教其研究借B。一公尿三线很借韩信为环像样省掉尿态会，在引光随机乔合只累在监分像计证中网子光形正多生位态计具耳尼尿。配置能系自物统使配共的n壁免、细片中添加额性步WTV r计部测形好诊超层扫9ESWCM E上9t机及于屈-CsI系配型露谱计转静态GZU基得的人可尿。Ca] c会型过位上场后式说明些将问题此两光10A展以下正确论半以计输谓，前原许然到发从那32-38还提KIC 民序 。没个一菌金氧C橱剂不二聚为 辅。用标LW的标是常米干化双哆利度灵对三标。能建通过包发得用的能温作最总。分释水序会、案等比具费设多度是中E表示。2pción的B， ain publication, we investigate the effect of nanographene oxide (nGO)-mediated hyperthermia in Saos-2 osteosarcoma cells. The results showed that nGO incorporation did not affect cell viability but caused a delay in cell proliferation. Additionally, 10 metabolites were found to have noticeable changes in cells incorporating nGO sheets, including decreased levels of amino acids, taurine, and creatine, and increased levels of phosphocholine and uridine/adenosine nucleotides. After NIR irradiation, cells exhibited further changes in the levels of glutamate and uridine nucleotides, as well as glycerophosphocholine and adenosine monophosphate. These findings demonstrate that the cellular metabolome is sensitive to nGO exposure and nGO-mediated hyperthermia, and NMR metabolomics is a powerful tool for investigating treatment responses.
作者：Monica Cicuendez, Joana Flores, Helena Oliveira, M. Teresa Portoles, Maria Vallet-Regi, Mercedes Vila, Lola F.Duarte
论文ID：2103.13122
分类：Tissues and Organs
分类简称：q-bio.TO
提交时间：2021-03-25